The purpose of this study was to observe histo-pathologic and histo-chemical reaction of the blood clot in alveolar : ockets following tooth extraction in dogs. Male Korean dogs, aged 8 months or more, were employed in this experiment.
The canine, third and fourth premolars of four quadrants were extracted under general anesthesia using Nembutal (pentobarbital sodium) injection and open drop ether:
The those animals were then sacrificed on 2nd, 3rd, 4th, 5th, 6th, 7th, 9th, 11th, 15th day after extraction respectively. The jaws were removed fixed in the solution of 1096N formalin, and decalcified in the 5% nitric acid and phloroglucin solution.
Each samples was stained various type of method which was Hematoxylin-eosin stain, P. T. A. H stain, Vangieson stain and Weigert stain.
The following results were obtained:
1. Clinically there was no evidence in delay of healing in any of the control sockets.
2. Epithelization required between three and nine days after the extraction of the tooth.
3. The formation of a blood clot was beginning of tissue regeneration in undisturbed healing of extraction wounds in animals.
4. At the end of the second day, fibroblasts had proliferated into this blood clot.
5. Bone regeneration was noted in the lower one third of the fundus of the socket on the sixth day.
6. The sockets were completely filled with new bone about fifteenth days after the extraction.
7. The sockets were filled with bone in fifteenth days and had reached a fairly stable condition at the end of. four weeks.
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